AREAS OF EXPERTISE
Blood testing, protein transfer by immunoblotting and analysis
By working in this discipline, I acquired a better understanding on the progress of clinical and translational studies that involved biological tissue banking. I froze the collected samples for future assays. Whether ELISA kits to validate markers during specific cellular processes or immunoblotting techniques to detect and identify antigens of interest. For each patient, demographic data and laboratory test results were compiled to generate statistics.
Sampling, clonal purification, anaerobic culture, taxonomy and analysis
The topic of my master’s thesis was to improve the phenotypic characterization of anaerobic bacteria in the periodontal pockets of different hosts. My work had an epidemiological focus, as I was interested in the spreading or routes of transmission of microorganisms. The conclusions drawn allowed me to describe a new bacterial species.
Throughout my career, I have worked in different fields of subsidized scientific research, some of which are described below. Today, I offer my linguistic services in every field of pure and applied science, upholding the same professional rigour as when I was a researcher.
Sampling, conformation polymorphism and Mendelian analysis
Marker-assisted selection is of primary interest in forest genomics, such as a phenotype for wood density. Well-designed screening will have a major impact on the timber industry. My work in this area involved comparing material from young shoots and adult trees. I used the single-strand conformation polymorphism technique to separate different alleles of the same gene. Progeny tests could then be done to describe the segregation from one generation to another according to the Mendelian principles of biological heredity.
Cellular transduction, animal models, cardiac imaging and analysis
My work in cellular biology allowed me to follow calcium signaling during in vitro events mimicking a heart attack. I was able to confirm that the presence of alcohol in moderate amounts is beneficial in limiting tissue damage to the cardiac muscle. Another noteworthy participation concerned early phases of pharmacokinetic and drug metabolism studies that required protocols using animals. In this context, I had succeeded in defining the best parameters for executing advanced imaging techniques, such as Doppler ultrasonography and x-ray computed tomography.
DNA, RNA, extraction, PCR, RT-PCR and analysis
I have increased my expertise in this specialized field since obtaining my certificate in genetic engineering. Many of the experiments I performed in this area concerned extraction and purification of nucleic acid molecules, as well as gene amplifications.